Released on = August 2, 2006, 4:53 pm

Press Release Author = Debra Thai

Industry = Biotech

Press Release Summary = As scientific research explores the role of b-Amyloid in
Alzheimer's Disease, the secretases whose cleavage activity produces b-Amyloid
fragments have become an important focus. One of the world's most comprehensive
providers of b-amyloid peptides, AnaSpec has introduced a new line of
amyloid-related secretase substrates, assay kits and inhibitors.

Press Release Body = Amyloid Precursor Protein, APP, a transmembrane glycoprotein of
about 770 amino acids, is cleaved by a-Secretase and b-Secretase to produce
C-terminal fragments that are 83 amino acids (C83) and 99-amino acids (C99) in
length, respectively. Subsequent cleavages of C83 and C99 by g-Secretase produce a
3-kD (p3) protein in the former and a 4-kD (b-amyloid) protein in the latter and a
CT57-59 in both fragments (reviewed in 1, 2). The 4-Kd consists of Ab which are 39
to 42 amino acids in length, with Ab 1-42 being the major component of amyloid
plaques which accumulates in neurons of Alzheimer's disease (AD) brain.
Consequently, b-Secretase is an important target for AD drug development.

FRET Secretase Substrates
AnaSpec provides fluorescence resonance energy transfer (FRET) substrates for use in
measuring b-Secretase activity. Upon cleavage of the FRET substrate by b-Secretase,
the quenched fluorescence of the donor is released, with the fluorescence signal
increasing with b-Secretase activity. Variations include a range of sequence
lengths and emission wavelengths.
· Wild-Type
· Swedish Double Mutation (K670N/M671L)

EnzoLyteT 520 ß-Secretase Assay Kit
· Ready-to-use assay kit for detection of b-Secretase V. 100 assays.

b -Secretase inhibitors
· High-purity peptides that inhibit b-Secretase activity.

a -Secretase Substrate
· A FRET-based a-Secretase substrate, which upon cleavage liberates the Mca
(7-Methoxycoumarin-4-acetic acid) fragment from the proximity quenching effect of
Dnp (2,4-dinitrophenyl), Abs/Em

Web Site = http://www.anaspec.com

Contact Details = 2149 O\'Toole Ave.
San Jose, CA 95131
408-452-5055
408-452-5059 fax
marketing@anaspec.com
info@anaspec.com
service@anaspec.com