Released on: June 25, 2008, 10:22 am

Press Release Author: AnaSpec Inc.

Industry: Biotech

Press Release Summary: Histone deacetylase (HDAC) enzymes modulate gene expression
through the deacetylation of lysine residues on histone proteins and act as
transcriptional repressors of genes.

Press Release Body: San Jose, CA - June 25, 2008
Histone deacetylase (HDAC) enzymes modulate gene expression through the
deacetylation of lysine residues on histone proteins and act as transcriptional
repressors of genes. Based on their role in cell cycling, apoptosis and
differentiation, HDACs have been chosen as therapeutic targets for the treatment of
cancer and neurodegenerative diseases.
AnaSpec has introduced the SensoLyteT 520 HDAC Activity Assay Kit, a novel assay kit
for HDAC detection (Ex/Em = 490 nm/520 nm) that is ideal for biological samples. The
shift in excitation/emission that is offered allows the SensoLyteTM 520 HDAC
Activity Assay Kit to avoid the autofluorescence interference from cell components
and test compounds that is commonly found in traditional HDAC assay kits.
The SensoLyteT 520 HDAC Activity Assay Kit provides a convenient, two-step
homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic
substrate. Without a time-consuming cell extraction step, this assay can be used to
measure HDAC activity directly in cell culture in a 96-well plate. This kit can also
be used for high throughput screening of HDAC inhibitors using extracts or purified
enzymes.
The kit contains: . HDAC 520 substrate . Deacetylated reference standard for
calibration . HeLa nuclear extract . Assay buffer . Cell lysis buffer . HDAC
developer . Trichostatin A (HDAC inhibitor)
AnaSpec also offers the SensoLyteT 440 HDAC Activity Assay Kit as a cost-effective
solution for moderate sensitivity. This kit provides a convenient, two-step
homogeneous procedure for measuring HDAC activity using an AMC-based fluorogenic
substrate (Ex/Em=354 nm/442 nm).
Company Info
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to
pharmaceutical, biotech, and academic research institutions throughout the world.
With a vision for innovation through synergy, AnaSpec focuses on three core
technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and
combinatorial chemistry.
For more information, visit www.anaspec.com



Web Site: http://www.anaspec.com

Contact Details: AnaSpec Inc.
2149 O\'Toole Ave.
San Jose, CA 95131
1-408-452-5055 (Tel)
1-408-452-5059 (Fax)
1-800-452-5530
ping@anaspec.com
www.anaspec.com